Peer reviewed articles

Tan, D., Gram, L. and Middelboe, M. 2014. Characterization of vibriophages and their interactions with the fish pathogen Vibrio anguillarum. Appl. Environ. Microbiol. 80 (10): 3128-3140

Prol Garcia, M.J., P.W.D’Alvise, A.M. Rygaard and L. Gram 2014. Biofilm formation is not a prerequisite for production of the antibacterial compound tropodithietic acid in Phaeobacter inhibens DSM17395. J. Appl. Microbiol 117:1592-1600

Rabe, P., Klapschinski. T.A., Brock, N.L., Citron, C.A., D’Alvise, P., Gram, L. and Dickschat, J.S. 2014. Synthesis and Bioactivity of Analogs of the Marine Antibiotic Tropodithietic Acid. Beilstein Journal of Organic Chemistry. Beilstein J. Org. Chem. 10:1796–1801

Tan, D., Dahl, A. and Middelboe M. (2015) Vibriophages differentially influence biofilm formation by Vibrio anguillarum strains. Applied and Environ. Microbiol. 81 (13):4489-4497.

Tan, D., Svenningsen, SL and Middelboe, M. (2015) Quorum sensing determines the choice of anti-phage defense strategy in Vibrio anguillarum. mBIO 6(3): 00627-1

Rasmussen, B.B, T. Grotkjær, P. W. D’Alvise, G. Yin, F. Zhang, R. Xu, B. Bunk, C. Spröer, M. Bentzon-Tilia and L. Gram 2016. Vibrio anguillarum is genetically and phenotypically unaffected by long-term continuous exposure to the antibacterial compound tropodithietic acid. Appl. Environ. Microbiol. 82:4802-4810

Grotkjær, T., P. D’Alvise, M. Bentzon-Tilia, K. Dierckens, P. Bossier and L. Gram 2016. Phaeobacter inhibens as probiotic bacteria in non-axenic Artemia and algae cultures. Aquaculture 462:64-69

Grotkjær, T., P. D’Alvise, N. Dourala, K.F. Nielsen and L. Gram 2016. Isolation of TDA-producing Phaeobacter strains from sea bass larval rearing units and their probiotic effect against pathogenic Vibrio spp. in Artemia cultures. System. Appl. Microbiol. 39:180-188

D’Alvise, P.W., C.B.W. Phippen, K.F. Nielsen and L. Gram 2016. Influence of iron on the production of the antibacterial compound tropodithietic acid and its non-inhibitory analog in Phaeobacter inhibens. Appl. Environ. Microbiol.  82:502-509

Rønneseth, A., P.W. D’Alvise, Ø. Tønnesen, G. Haugland, D. Castillo, T. Grotkjær, K. Engell-Sørensen, L. Nørremark, Ø. Bergh, H. I. Wergeland and L. Gram 2017. Comparative assessment of Vibrio virulence in marine fish larvae. J. Fish Dis.(in press)  DOI: 10.1111/jfd.12612

Castillo, D., P.W D Alvise, R. Xu, F. Zhang. M. Middelboe and L. Gram 2017. Comparative genome analyses of Vibrio anguillarum strains reveal a link with pathogenicity traits. mSystems 2: e00001-17

Kalatzis, P., Rørbo, N.I., Castillo, D., Mauritzen, J.J., Jørgensen, J., Kokkari, C., Zhang, F. Katharios, P. and  M. Middelboe 2017. Stumbling across the same phage: Comparative genomics of widespread temperate phages infecting the fish pathogen Vibrio anguillarumViruses 9:122; doi:10.3390/v9050122.

Katharios, P., Kalatzis, P.G., Kokkari, K., Sarropoulou, E. and M.  Middelboe 2017. Isolation and characterization of an N4-like lytic bacteriophage infecting Vibrio splendidus, a pathogen of fish and bivalves. PLoS ONE 12:e0190083. https://doi. org/10.1371/journal.pone.0190083. 

Kalatzis, P., Castillo, D., Katharios, P. and M. Middelboe 2018. Bacteriophage interactions with marine pathogeniuc vibrios: Implications for phage therapy (review). Antibiotics 7(1), 15; doi:10.3390/antibiotics7010015

Dittmann, K.K., B.B. Rasmussen, M. Castex, L. Gram, M. Bentzon-Tilia 2017. The aquaculture microbiome at the centre of business creation (editorial). Microb. Biotechnol. doi:10.1111/1751-7915.12877

Rasmussen, B.B., K.E. Erner, M. Bentzon-Tilia and L. Gram 2018. Effect of TDA-producing Phaeobacter inhibens on the fish pathogen Vibrio anguillarum in non-axenic algae and copepod systems. Mar. Biotechnol.  doi: 10.1111/1751-7915.13275

Rørbo, N., A. Rønneseth, P.G. Kalatzis, B.B. Rasmussen, K. Engell-Sørensen, H.P. Kleppen, H.I. Wergeland, L. Gram and M. Middelboe 2018. Potential of phage therapy in preventing Vibrio anguillarum infections in cod and turbot larvae. Antibiotics 16;7(2). pii: E42. doi: 10.3390/antibiotics7020042.


Genome Announcements 

Romero, J., Higuera, G. Gajardo, F., Castillo, D., Middelboe, M., Garcia, K., Ramirez, C., and Espejo, R. (2014)  Complete genome sequence of Vibrio anguillarum phage CHOED successfully used for phage therapy in aquaculture" GenomeA 2 (4): e0009-14.

Castillo, D., P. D´Alvise, M. Middelboe, L. Gram, S. Liu, P. Kalatzis, C. Kokkari, and P. Katharios. 2015 Draft genome sequences of the fish pathogen Vibrio harveyi strains VH2 and VH5. Genome Announcements 3:e01062-15; doi:10.1128/genomeA.01062-15

Castillo, D., P. D Alvise, P. Kalatzis, C. Kokkari, M. Middelboe, L. Gram, S. Liu and P. Katharios. 2015. Draft genome sequences of Vibrio alginolyticus strains V1 and V2, opportunistic marine pathogens. Genome Announcements 3:e00729-15; doi:10.1128/genomeA.00729-15

Castillo, D., J. Woo Jun, P. D´Alvise, M. Middelboe, L. Gram, S. Liu, and P. Katharios 2015. Draft genome sequence of Vibrio parahaemolyticus VH3 isolated from aquaculture environment in Greece. Genome Announcements 3:e00731-15; doi:10.1128/genomeA.00731-15


Conference lectures

D’Alvise, P.W., S.Lillebø, H.Wergeland, K.F.Nielsen, Ø.Bergh and L.Gram. 2013. Control of bacterial disease in cultures of marine larvae and live feed organisms by a probiotic bacterium. Presentation at LARVI, Ghent, Belgium

Demeng, T. and M.Middelboe  2013. Phage KVP40 Enhance Biofilm Formation in Vibrio anguillarum (PF430-3). Oral presentation at International Biofilm Course, University of Copenhagen

Tan, D., Svenningsen, SL., Middelboe, M. Two alternating defense strategies: 1) Spatial refuge and 2) Quorum Sensing induced down-regulation of OmpK receptor. Poster at EMBO Meeting: Viruses of Microbes, Zurich 2014

Tan, D., Svenningsen, SL., Middelboe, M. Quorum sensing determines the choice of anti-phage defense strategy in Vibrio anguillarum. Poster at Bacterophages 2015 meeting. London.

Middelboe, M. Examination of phage resistance mechanisms in marine Vibrio anguillarum. Seminar at Coastal Pathogen Group, University of Exeter, UK. 2015

Middelboe, M. Phage-host interactions in marine fish pathogens. Seminar at Niels Bohr Institute, University of Copenhagen. 2015

Middelboe, M. Virus-bacteria interactions: Nanoscale processes with global scale implications. Seminar at Institute for Chemistry and Biology of the Marine Environment (ICBM), University Oldenburg, Germany, 2015

Castillo, D.: Distribution of prophage-encoded properties in Vibrios. Workshop on “Phage-Vibrio interactions”. Massachusetts Institute of Technology, 26-28 April 2016

Middelboe, M.: Phage-host interactions in fish pathogens: Examination of phage resistance. Workshop on “Phage-Vibrio interactions”. Massachusetts Institute of Technology, 26-28 April 2016

Kalatzis, P.: Phage control of Vibrio and development of resistance. Workshop on “Phage-Vibrio interactions”. Massachusetts Institute of Technology, 26-28 April 2016

Rørbo, N.: Genomic analysis of Vibriophages. Workshop on “Phage-Vibrio interactions”. Massachusetts Institute of Technology, 26-28 April 2016

Middelboe, Tan & Svenningsen: Anti-phage defense strategies in the marine fish pathogen Vibrio anguillarum. Aquatic Virus Workshop, Plymouth July 2016.

Middelboe, M.: Antiphage defense strategies in the fish pathogens Vibrio anguillarum and Flavobacterium psychrophilum: implications for the use of phages in disease control. EMBO conference: Viruses of Microbes. Liverpool, July 2016

Middelboe, M.: Use of phages for pathogen control in aquaculture. PHAGES Meeting, Oxford September 2016.


Grotkær, T., P.W. D'Alvise and L.Gram 2013. Use of Phaeobacter inhibens as a probiont targeting Vibrio in Artemia cultures. Poster at Danish Society for Microbiology Yearly Meeting, Copenhagen, Denmark.

Demeng, T., L.Gram and M.Middelboe 2013. Characterization of Vibriophages and Their Interactions with the Fish Pathogen Vibrio anguillarum. Poster presented at PhD day at the Department of Biology, University of Copenhagen